Carcinogenesis, Teratogenesis & Mutagenesis ›› 1999, Vol. 11 ›› Issue (2): 80-085.doi: 10.3969/j.issn.1004-616x.1999.02.006

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THE EXPERIMENTAL STUDY OF PRECANCEROUS LESION OF PANCREATIC ACINAR CELLULAR CANCER IN RATS INDUCED BY AZASERINE AND THE DETECTION OF K2RAS MUTATION

Xu Feng1 , Cao Wei2 , Shou Nanhai1 ,J iang Xihong1 ,Xu Kesun1   

  1. 1 Department of General S urgery , The A f f iliated Hospital of S handong Medical University , 2Department of Pharmacology , S handong Medical University 　Jinan
  • Received:1998-01-17 Revised:1998-06-16 Online:1999-03-30 Published:1999-03-30

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Abstract: To set up the animal model of precancerous lesion of rat’s pancreatic acinar cellular cancer , learn its biological feature and K2ras gene code 12 mutation. Male Wistar rat s were injected Azaserine 30mg/ kg (ip) . 4 months later , rat s’pancreas were autopsied and K2ras gene code 12 mutation were detected by PCR2SSCP. The incidence of precancreous lesion of rat s’pancreatic acinar cellular cancer was 42. 1 %. Azaserine could induce precancerous lesion of rat s’pancreatic acinar cancer successfully , K2ras gene code 12 mutation was early change during this process.

Key words: Azaserine, Pancreatic neoplasm, K-ras gene